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| Ultimate™ ORFCard for Clone ID IOH10269 | |
|---|---|
| Gene Information | |
| Clone ID: | IOH10269 |
| Organism: | Homo sapiens |
| Matching Nucleotide Accession: | ENST00000362029 |
| Category: | 112950 |
| Comment: | HS.301756 |
| Related Accession(s): | AL139289|Alignment || AF521562|Alignment || AV684532|Alignment || BC010019|Alignment || BC010543|Alignment || BG620658|Alignment || CB161514|Alignment |
| Gene Name: | mediator of RNA polymerase II transcription, subunit 8 homolog (yeast) |
| Gene Definition: | mediator complex subunit 8 [Source:HGNC Symbol;Acc:19971] |
| Gene Symbol: | MED8 |
| Summary: | This gene encodes a protein that is one of more than 20 subunits of the mediator complex, first identified in S. cerevisiae, that is required for activation of transcription. The product of this gene also interacts with elongins B and C, and CUL2 and RBX1, to reconstitute a ubiquitin ligase. Five alternative transcripts encoding four isoforms have been described. |
| Expression: | Sage Tag Expression || Virtual Northern || Digital Expression Profile |
| Transcript Variant 1: | This variant (1) is the longest transcript but encodes the shortest isoform (1). Variants 1 and 4 encode the same isoform (1). |
| Transcript Variant 2: | This variant (2) uses alternate splice sites in the 5' and 3' coding regions, compared to variant 1. The resulting protein (isoform 2) is longer and has a distinct C-terminus, compared to isoform 1. |
| Transcript Variant 3: | This variant (3) uses alternate splice sites in the 5' and 3' coding regions, compared to variant 1. The resulting protein (isoform 3) is the longest isoform, and differs in the N- and C-termini, compared to isoform 1. |
| Transcript Variant 4: | This variant (4) differs in the 3' UTR compared to variant 1. Variants 1 and 4 encode the same isoform (1). |
| Transcript Variant 5: | This variant (5) uses alternate splice sites in the 5' coding region and differs in the 3' UTR, compared to variant 1. The resulting protein (isoform 4) is longer, and has a longer and distinct N-terminus, compared to isoform 1. |
| mRNA Record: | NM_001001651|Alignment || NM_001001653|Alignment || NM_001001654|Alignment || NM_052877|Alignment || NM_201542|Alignment |
| GO Category: biological process | regulation of transcription, DNA-dependent (GO:0006355) transcription (GO:0006350) |
| GO Category: cellular component | nucleus (GO:0005634) |
| GO Category: molecular function | receptor activity (GO:0004872) |
| References: | GRIF: 112950 | PUBMED: MED8 |
| ORF Information | |
| ORF length (bp): | 639 |
| Sequence: | Nucleotide || Peptide || Translation || Quality Scores || Quality Scores with Sequence |
| Clone Information | |
| Collection Name: | Ultimate ORF Clones |
| Collection Type: | ORF Gateway™ Entry |
| Vector Name: | pENTR(tm)221 |
| Vector Antibiotic: | Kanamycin |
| Host Name: | E.coli |
| Protein | |
| Protein Accession: | CAI23381|Alignment || CAI23382|Alignment || AAM76709|Alignment || AAH10019|Alignment || AAH10543|Alignment || Q96G25|Alignment |
| Protein Record: | NP_001001651|Alignment || NP_001001653|Alignment || NP_001001654|Alignment || NP_443109|Alignment || NP_963836|Alignment |
| Physical Properties: | (aa) || 0.0 (MW) || 0.0 (pI) |
| Protease Digestion: | Trypsin | Lys-C | Arg-C | Asp-N | V8-bicarb | V8-phosph | Chymotrypsin | CNBr |
| Predicted Secondary Structure: | View Secondary Structure |
| Protein Model Search: | Swiss-Model BLAST |
| OMIM: | 607956 |
| Product: | mediator of RNA polymerase II transcription subunit MED8 isoform 4 |
| Human Proto Array: | ProtoArray Human Protein Microarray nc v1.0 |
| SNP Information | |
| SNP: | All rs in gene region | rs in coding region only | rs with heterozygosity only |
| SNP Map to: | Protein |
| Genomic Link | |
| LocusLink ID: | 112950 |
| Unigene ID: | Hs.301756 |
| Genome Alignment: | Map to Human Genome using BLAT || Map to Ensembl Genome Browser |
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Disclaimer: Invitrogen™ Corporation provides an evolving clone collection where each gene represented is identified as containing a complete open reading frame, based on a dynamic source of bioinformatic information contained within GenBank. GenBank is a genetic sequence database, which contains an annotated collection of all publicly available DNA sequences. Ultimate™ ORF Clones are typically derived from cDNA clones believed to contain a complete open reading frame, according to the most current GenBank update. Please note, subsequent GenBank updates may show some of these cDNA clones to contain a partial sequence, a non-coding sequence or other artifacts. Quarterly, Invitrogen™ Corporation will update the Ultimate™ ORF Clone Collection, by adding, deleting, or modifying the bioinformatic information to more accurately reflect the most current scientific information associated with each of the clones represented in the collection. It is highly recommended that the purchaser carefully evaluate the ORF clone sequence prior to purchase. It is the purchaser’s responsibility to check whether the sequence associated with any particular clone meets his/her scientific requirement prior to purchase. Invitrogen makes no warranties, express or implied, that the manufacture, use, importation, or sale of the gene contained within any Ultimate™ ORF clone will not infringe any third party intellectual property right. | |