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| Ultimate™ ORFCard for Clone ID IOH10191 | |
|---|---|
| Gene Information | |
| Clone ID: | IOH10191 |
| Organism: | Homo sapiens |
| Matching Nucleotide Accession: | BC009108.1|Alignment |
| Category: | 55388 |
| Comment: | HS.198363 |
| Related Accession(s): | AL138764|Alignment || AL355355|Alignment || AB042719|Alignment || AF119869|Alignment || AK055695|Alignment || AL136840|Alignment || BC004876|Alignment || BC009108|Alignment |
| Gene Name: | MCM10 minichromosome maintenance deficient 10 (S. cerevisiae) |
| Gene Definition: | Homo sapiens, cDNA clone IMAGE:3451214 |
| Gene Symbol: | MCM10 |
| Summary: | The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and it may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2. It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner. Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding. Alternatively spliced transcript variants encoding distinct isoforms have been identified. |
| Expression: | Sage Tag Expression || Virtual Northern || Digital Expression Profile |
| Transcript Variant 1: | This variant (1) uses a different splice site, compared to variant 2, that results in a longer isoform (1). Though this variant is supported by EST data, the existence of its encoded isoform has not been experimentally verified. |
| Transcript Variant 2: | This variant (2) encodes the predominant isoform (2). |
| mRNA Record: | NM_018518|Alignment || NM_182751|Alignment |
| References: | GRIF: 55388 | PUBMED: MCM10 |
| ORF Information | |
| ORF length (bp): | 1062 |
| Sequence: | Nucleotide || Peptide || Translation || Quality Scores || Quality Scores with Sequence |
| Clone Information | |
| Collection Name: | Ultimate ORF Clones |
| Collection Type: | ORF Gateway™ Entry |
| Vector Name: | pENTR(tm)221 |
| Vector Antibiotic: | Kanamycin |
| Host Name: | E.coli |
| Protein | |
| Protein Accession: | CAI12912|Alignment || CAI12913|Alignment || CAI16557|Alignment || CAI16558|Alignment || BAB18723|Alignment || AAF69623|Alignment || BAB70988|Alignment || CAB66774|Alignment || AAH04876|Alignment || AAH09108|Alignment |
| Protein Record: | NP_060988|Alignment || NP_877428|Alignment |
| Physical Properties: | (aa) || 0.0 (MW) || 0.0 (pI) |
| Protease Digestion: | Trypsin | Lys-C | Arg-C | Asp-N | V8-bicarb | V8-phosph | Chymotrypsin | CNBr |
| Predicted Secondary Structure: | View Secondary Structure |
| Protein Model Search: | Swiss-Model BLAST |
| OMIM: | 609357 |
| Product: | minichromosome maintenance protein 10 isoform 2 |
| Human Proto Array: | ProtoArray Human Protein Microarray nc v1.0 |
| SNP Information | |
| SNP: | All rs in gene region | rs in coding region only | rs with heterozygosity only |
| SNP Map to: | Protein |
| Genomic Link | |
| LocusLink ID: | 55388 |
| Unigene ID: | Hs.198363 |
| Genome Alignment: | Map to Human Genome using BLAT || Map to Ensembl Genome Browser |
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Disclaimer: Invitrogen™ Corporation provides an evolving clone collection where each gene represented is identified as containing a complete open reading frame, based on a dynamic source of bioinformatic information contained within GenBank. GenBank is a genetic sequence database, which contains an annotated collection of all publicly available DNA sequences. Ultimate™ ORF Clones are typically derived from cDNA clones believed to contain a complete open reading frame, according to the most current GenBank update. Please note, subsequent GenBank updates may show some of these cDNA clones to contain a partial sequence, a non-coding sequence or other artifacts. Quarterly, Invitrogen™ Corporation will update the Ultimate™ ORF Clone Collection, by adding, deleting, or modifying the bioinformatic information to more accurately reflect the most current scientific information associated with each of the clones represented in the collection. It is highly recommended that the purchaser carefully evaluate the ORF clone sequence prior to purchase. It is the purchaser’s responsibility to check whether the sequence associated with any particular clone meets his/her scientific requirement prior to purchase. Invitrogen makes no warranties, express or implied, that the manufacture, use, importation, or sale of the gene contained within any Ultimate™ ORF clone will not infringe any third party intellectual property right. | |